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Brain-Choroid Plexus Chemistry Changes Linkable to Caffeine Ingestion and Accompanying Short-Term Memory Disturbances in Experimental Models

Received: 31 August 2017     Accepted: 25 September 2017     Published: 26 October 2017
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Abstract

A number of literatures have reported that caffeine could have negative effects on mental health and brain structures and chemistry. There are also reported positive effects of caffeine, such as improvement in memory, especially for those who consume caffeine from its natural sources. Sixty male juvenile Wistar rats were grouped into six (6) groups of 10 rats in each. The groups were labeled A-F with group A being the control group and groups B, C, D, E, and F being the treated groups. This investigation complements our previous efforts to study the effects of caffeine on the choroid plexus microscopic structure, which is involved in the production of the cerebrospinal fluids. Caffeine alters the activities of key enzymes that are associated with the production of CSF. This showed that the structural changes have observable alterations of the brain chemistry. Also, memory, a major attribute of mental power was tested in the same models; results showed that caffeine affected the short term memories. The effects of caffeine was studied in models that ingested caffeine solely and in other modeled after human use by consuming it with honey, a natural antioxidant rich sweetener. In this study, it was observed that the long-term consumption and high amount of caffeine increased the expression of carbonic anhydrase enzyme in the choroid plexus, this enzyme plays a major role in the production of CSF. In the caffeine-treated rats, there was also increased expression of Na+/K+-ATPase which may be associated with changes in the choroid epithelial cells. Honey improved the glutathione peroxidase level. Results showed the use of caffeine with honey had positive effects against the observed effects caused by ingestion of caffeine only. This also showed that caffeine use effects would vary in users of pure caffeine as mere drugs; and habitual consumers as beverages. Generally, evidences lead us to conclude that alterations in enzymes activities are associated with choroid plexus changes that could affect CSF production. These effects are also associated with behavioural changes.

Published in American Journal of Laboratory Medicine (Volume 2, Issue 5)
DOI 10.11648/j.ajlm.20170205.15
Page(s) 104-111
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Brain Chemistry, Caffeine, Choroid, Honey, Antioxidant, Cerebrospinal Fluid, Memory

References
[1] Akanmu MA, Echeverry C, Rivera F, and Dajas F, (2009). Antioxidant and neuroprotective effects of Nigerian honey, in Proceedings of the Nueroscience Meeting Planner, Washington, DC, USA.
[2] Albertson TE, Joy RM, and Stark LG, (1983). Caffeine modification of kindled amygdaloid seizures, Pharmacology Biochemical Behaviour, 19, 2: 339-343.
[3] Alzahrani HA, Alsabehi R, Boukraˆa L, Abdellah F, Bellik Y, and Bakhotmah BA, (2012). Antibacterial and antioxidant potency of floral honeys from different botanical and geographical origins, Molecules, 17, 9: 10540–10549.
[4] Amy EJ and Carlos ME, (1996). Medical uses of honey, Revista Biomédica, 7: 43–49.
[5] Angelucci ME, Cesario C, Hiroi RH, Rosalen PL, and Da Cunha C, (2002): Effects of caffeine on learning and memory in rats tested in the Morris water maze. Brazilian journal of medical and biological research, 35, 10:1201-1208.
[6] Ault B, Olney MA, Joyner JL, Boyer CE, Notrica MA, Soroko FE, and Wang CM, (1987). Pro-convulsant actions of theophylline and caffeine in the hippocampus, implications for the management of temporal lobe epilepsy. Journal of Brain Res, 426, 1: 93-102.
[7] Awatramani R., Soriano P, Rodriguez C, Mai JJ, and Dymecki SM (2003). Cryptic boundaries in roof plate and choroid plexus identified by intersectional geneactivation. Nat. Genet journal, 35: 70–75.
[8] Back SA, Craig A, Luo NL, Ren J, Akundi RS, Ribeiro I, and Rivkees SA, (2006). Protective effects of caffeine on chronic hypoxia-induced perinatal white matter injury. Annals of neurology, 60, 6: 696-705.
[9] Beer and Alan E, (2009): Is Your Body Baby Friendly? AJR Publishing, Hialeah, Florida: 258.
[10] Bolos M, Spuch C, Ordoñez-Gutierrez L, Wandosell F, Ferrer I, and Carro E, (2013). Neurogenic effects of β-amyloid in the choroid plexus epithelial cells in Alzheimer’s disease, Journal of Cell. Mol. Life Sci, 70:2787–2797.
[11] Brightman MW, and Reese TS, (1969). Junctions between intimately apposed cell membranes in the vertebrate brain, Journal of Cell, 40, 648–677.
[12] Cai M, Shin BY, Kim DH, (2011). Neuroprotective effects of a traditional herbal prescription on transient cerebral global ischemia in gerbils, Journal of Ethnopharmacology, 138, 3: 723–730.
[13] Cantarelli MA, Pellerano RG, Marchevsky EJ, and Camiña JM, (2008). Quality of honey from Argentina study of chemical composition and trace elements, Journal of the Argentine Chemical Society, 96, 2: 33–41.
[14] Cardiff R, Miller CH, and Munn RJ (2014). Manual Haematoxylin and Eosin staining of mouse tissue sections, Article in Cold Spring Harbor Protocols, 6: 561-565.
[15] Carlos AU, David H, and Carmen G, (2011). Role of honey polyphenols in health, Journal of Api Product and Api Medical Science, 3, 4: 141–159.
[16] Carson MJ, Thrash JC, and Walter B, (2006). The cellular response in neuroinflammation: the role of leukocytes, microglia and astrocytes in neuronal death and survival, Clinical Neuroscience Research, 6, 5, 237–245.
[17] Chute RK, Deogade NG, and Kawale M, (2010). Antimicrobial activity of Indian honey against clinical isolates, Asiatic Journal of Biotechnology Resources, 1: 35–38.
[18] Crisan Elena and Benbadis SR, (2016). Ventricles of the brain; Updated in June 30.
[19] Currle, DS, Cheng X, Hsu CM, and Monuki ES, (2005). Direct and indirect roles of CNS dorsal midline cells in choroid plexus epithelia formation, Journal of Development, 132: 3549–3559.
[20] El Yacoubi M, Ledent C, Parmentier M, Costentin J, and Vaugeois JM, (2003): Caffeine reduces hypnotic effects of alcohol through adenosine A2A receptor blockade. Neuropharmacology, 45, 7: 977-985.
[21] Esposito E, Rotilio D, di Matteo V, di Giulio C, Cacchio M, and Algeri S, (2002). A review of specific dietary antioxidants and the effects on biochemical mechanisms related to neurodegenerative processes, Journal of Neurobiology of Aging, 23, 5: 719–735.
[22] Field AS, Laurienti PJ, Yen YF, Burdette JH, and Moody DM, (2003). Dietary caffeine consumption and withdrawal: confounding variables in quantitative cerebral perfusion studies? Radiology, 227, 1:129-135.
[23] Francis Agyemang-Yeboah and Sylvester Yaw Oppong, (2013). Caffeine: The wonder compound, chemistry and properties” 27-37 ISBN: 978-81-308-0521-4.
[24] Frank-Cannon TC, Alto LT, Mc Alpine FE, and Tansey MG, (2009). Does neuroinflammation fan the flame in neurodegenerative diseases? Molecular Neurodegeneration, 4, 1, 47.
[25] Fredholm BB, Battig K, Holmen J, Nehlig A, and Zvartau EE (1999): Actions of caffeine in the brain with special reference to factors that contribute to its widespread use. Pharmacological reviews, 51, 1:83-133.
[26] French VM, Cooper RA, and Molan PC, (2005). The antibacterial activity of honey against coagulase-negative staphylococci,” Journal of Antimicrobial Chemotherapy, 56, 1: 228– 231.
[27] Georgiev V, Johansson B, and Fredholm BB, (1993): Long-term caffeine treatment leads to a decreased susceptibility to NMDA-induced clonic seizures in mice without changes in adenosine A1 receptor number. Brain Res, 612:271-277.
[28] Howell LL, Coffin VL, Spealman RD, (1997): Behavioral and physiological effects of xanthines in nonhuman primates. Journal of Psychopharmacology, 129, 1:1-14.
[29] Hunter NL and Dymecki SM. (2007). Molecularly and temporally separable lineages form the hind brain roof plate and contribute differentially to the choroid plexus. Journal of Development 134: 3449–3460.
[30] Imayoshi I, Shimogori T, Ohtsuka T, and Kageyama R, (2008). Hesgenes and neurogenin regulatenon-neural versus neural fate specification in the dorsal telencephalic midline. Development, 135: 2531–2541.
[31] Islam A, Khalil MI, and Islam MN, (2012). Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year, BMC Complementary and Alternative Medicine, 12, 177: 1-9.
[32] Itokazu Y, Kitada M, Dezawa M, Mizoguchi A, Matsumoto N, and Shimizu A, (2006). Choroid plexus ependymal cells host neural progenitor cells in the rat. Glia 53: 32–42.
[33] Johansson B, Georgiev V, Kuosmanen T, Fredholm BB, (1996). Long-term treatment with some methylxanthines decreases the susceptibility to bicuculline- and pentylenetetrazol-induced seizures in mice. Relationship to c-fos expression and receptor binding. Eur J Neurosci, 8, 12: 2447-2458.
[34] Khalil MI, Alam N, Moniruzzaman M, Sulaiman SA, and Gan SH, (2011). Phenolic acid composition and antioxidant properties of Malaysian honeys, Journal of Food Science, 76, 6, C921–C928.
[35] Khalil MI, Moniruzzaman M, Boukraˆa L, (2012). Physicochemical and antioxidant properties of Algerian honey, Molecules, vol. 17, no. 9, pp. 11199–11215.
[36] Lau FC, Shukitt-Hale B, and Joseph JA, (2005). The beneficial effects of fruit polyphenols on brain aging, Neurobiology of Aging, 26: S128–S132.
[37] Mahaneem Mohamed, Siti Amrah Sulaiman, Hasnan Jaafar and Kuttulebbai Nainamohamed Salam Sirajudeen, (2011). Int. J. Mol. Sci., 12: 5508-5521; ISSN 1422-0067.
[38] Mandal M and Jaganathan SK, (2009). Antiproliferative effects of honey and of its polyphenols: a review, Journal of Biomedicine and Biotechnology, 2009, 830616: 13.
[39] Manyi-Loh CE, Clarke AM, and Ndip RN, (2011). An overview of honey: therapeutic properties and contribution in nutrition and human health, African Journal of Microbiology Research, 5, 8: 844–852.
[40] Mato I, Huidobro JF, Simal-Lozano J, and Sancho MT, (2003). Significance of nonaromatic organic acids in honey, Journal of Food Protection, 66, 12: 2371–2376,
[41] Nehlig A, (1999): Are we dependent upon coffee and caffeine? A review on human and animal data. Neuroscience and biobehavioral reviews, 23, 4: 563-576.
[42] Oyefuga OH, Ajani EO, Salau BA, Agboola F, and Adebawo OO, (2012). Honey consumption and its anti-ageing potency in white Wister albino rats, Scholarly Journal of Biological Science, 1, 2: 15–19.
[43] Oyekunle OA, Akanmu MA, and Ogundeji TP, (2010). Evaluation of anxiolytic and novelty induced behaviours following bee-honey consumption in rats, Journal of Neuroscience and Behavioural Health, 2, 4: 38–43.
[44] Pellerano RG, Marchevsky EJ, and Cami˜na JM, (2008). Quality of honey from Argentina, study of chemical composition and trace elements, Journal of the Argentine Chemical Society, 96, 2: 33–41.
[45] Pradip Sarkar K, (2002). A Quick Assay for Na+/ K+-ATPase Specific Activity, Verlag der Zeitschrit fur Naturforschung, Tubingen, 57: 562-564.
[46] Rudolphi KA, Keil M, Fastbom J, Fredholm BB, (1989). Ischaemic damage in gerbil hippocampus is reduced following upregulation of adenosine (A1) receptors by caffeine treatment. Neurosci Letter, 103, 3:275-280.
[47] Sammataro D and Avitabile A (2011). The Beekeeper’s Handbook (4th ed.). Ithaca, NY: Cornell University Press.
[48] Sato T and Miyata G, (2000). The nutraceutical benefit, part III: honey, Nutrition, 16, 6: 468–469.
[49] Schlager N, Weisblatt J, Newton D, Montney E, Charles B, Thomson G and Detroit MI, (2006). Chemical Compounds, 1: 140-1.
[50] Schmitt-Schillig S, Schaffer S, Weber CC, Eckert GP, and M¨uller WE, “Flavonoids and the aging brain,” Journal of Physiology and Pharmacology, vol. 56, no. 1, pp. 23–36, 2005.
[51] Speake T and Brown PD, (2004). Ion channels in epithelial cells of the choroid plexus isolated from the lateral ventricle of rat brain. Brain Res. 1005: 60–66.
[52] Strange but True Science, (2014). Publications International, Ltd.: Lincolnwood, IL: 92.
[53] Strazielle N and Ghersi-Egea JF, (2000). Journal Neuropathol Exp Neurol, 59, 7: 561-74, INSERM U433, Faculte de Medicine Laennec, Lyon. France.
[54] Suchocki John, (2001). Conceptual Chemistry- Understanding Our World of Atoms and Molecules; Addison Wesley: San Francisco, California: 438-462.
[55] Sutherland GR, Peeling J, Lesiuk HJ, Brownstone RM, Rydzy M, Saunders JK, and Geiger JD, (1991). The effects of caffeine on ischemic neuronal injury as determined by magnetic resonance imaging and histopathology. Neuroscience, 42, 1:171-182.
[56] Turner CP, Seli M, Ment L, Stewart W, Yan H, Johansson B, Fredholm BB, Blackburn M, Rivkees SA, (2003). A1 adenosine receptors mediate hypoxia-induced ventriculomegaly. Proceedings of the National Academy of Sciences of the United States of America, 100, 20:11718-11722.
[57] Universidad de Barcelona, (2010). Coffee and a sweet treat to think better? Caffeine and glucose combined improves the efficiency of brain activity, Science Daily, Retrieved April 30, 2017 from www.sciencedaily.com/releases/2010/11/101123101751.htm
[58] Volpe JJ, (2001). Neurobiology of periventricular leukomalacia in the premature infant. Pediatric research, 50, 5: 553-562.
[59] Von Lubitz DK, Dambrosia JM, Kempski O, and Redmond DJ, (1988). Cyclohexyl adenosine protects against neuronal death following ischemia in the CA1 region of gerbil hippocampus. Stroke, 19, 9:1133-1139.
[60] Weinberg BA, and Bealer BK (2001). The world of caffeine, New York & London: Routledge. ISBN 0-415-92722-6.
[61] White Jr JW, (1980). Detection of honey adulteration by carbohydrate analysis, Journal of the Association of Official Analytical Chemists, 63, 1: 11–18.
[62] White JW, (1962). Composition of American Honeys, Agricultural Research Service USDA, Washington, DC, USA.
[63] Young SN and Gauthier S, (1981). Effect of tryptophan administration on tryptophan, 5-hydroxyindoleacetic acid and indoleacetic acid in human lumbar and cisternal cerebrospinal fluid, Journal of Neurology Neurosurgery and Psychiatry, 44, 4: 323–328.
[64] Z´arraga-Galindo N, Vergara-Arag´on P, Rosales-Mel´endez S, (2011). Effects of bee products on pentylenetetrazole-induced seizures in the rat, Proceedings of the Western Pharmacological Society, 54: 32–39.
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    Owolabi Joshua Oladele, Olatunji Sunday Yinka, Olanrewaju John Afees, Jimoh-Enesi Queen Amina. (2017). Brain-Choroid Plexus Chemistry Changes Linkable to Caffeine Ingestion and Accompanying Short-Term Memory Disturbances in Experimental Models. American Journal of Laboratory Medicine, 2(5), 104-111. https://doi.org/10.11648/j.ajlm.20170205.15

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    Owolabi Joshua Oladele; Olatunji Sunday Yinka; Olanrewaju John Afees; Jimoh-Enesi Queen Amina. Brain-Choroid Plexus Chemistry Changes Linkable to Caffeine Ingestion and Accompanying Short-Term Memory Disturbances in Experimental Models. Am. J. Lab. Med. 2017, 2(5), 104-111. doi: 10.11648/j.ajlm.20170205.15

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    AMA Style

    Owolabi Joshua Oladele, Olatunji Sunday Yinka, Olanrewaju John Afees, Jimoh-Enesi Queen Amina. Brain-Choroid Plexus Chemistry Changes Linkable to Caffeine Ingestion and Accompanying Short-Term Memory Disturbances in Experimental Models. Am J Lab Med. 2017;2(5):104-111. doi: 10.11648/j.ajlm.20170205.15

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  • @article{10.11648/j.ajlm.20170205.15,
      author = {Owolabi Joshua Oladele and Olatunji Sunday Yinka and Olanrewaju John Afees and Jimoh-Enesi Queen Amina},
      title = {Brain-Choroid Plexus Chemistry Changes Linkable to Caffeine Ingestion and Accompanying Short-Term Memory Disturbances in Experimental Models},
      journal = {American Journal of Laboratory Medicine},
      volume = {2},
      number = {5},
      pages = {104-111},
      doi = {10.11648/j.ajlm.20170205.15},
      url = {https://doi.org/10.11648/j.ajlm.20170205.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20170205.15},
      abstract = {A number of literatures have reported that caffeine could have negative effects on mental health and brain structures and chemistry. There are also reported positive effects of caffeine, such as improvement in memory, especially for those who consume caffeine from its natural sources. Sixty male juvenile Wistar rats were grouped into six (6) groups of 10 rats in each. The groups were labeled A-F with group A being the control group and groups B, C, D, E, and F being the treated groups. This investigation complements our previous efforts to study the effects of caffeine on the choroid plexus microscopic structure, which is involved in the production of the cerebrospinal fluids. Caffeine alters the activities of key enzymes that are associated with the production of CSF. This showed that the structural changes have observable alterations of the brain chemistry. Also, memory, a major attribute of mental power was tested in the same models; results showed that caffeine affected the short term memories. The effects of caffeine was studied in models that ingested caffeine solely and in other modeled after human use by consuming it with honey, a natural antioxidant rich sweetener. In this study, it was observed that the long-term consumption and high amount of caffeine increased the expression of carbonic anhydrase enzyme in the choroid plexus, this enzyme plays a major role in the production of CSF. In the caffeine-treated rats, there was also increased expression of Na+/K+-ATPase which may be associated with changes in the choroid epithelial cells. Honey improved the glutathione peroxidase level. Results showed the use of caffeine with honey had positive effects against the observed effects caused by ingestion of caffeine only. This also showed that caffeine use effects would vary in users of pure caffeine as mere drugs; and habitual consumers as beverages. Generally, evidences lead us to conclude that alterations in enzymes activities are associated with choroid plexus changes that could affect CSF production. These effects are also associated with behavioural changes.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Brain-Choroid Plexus Chemistry Changes Linkable to Caffeine Ingestion and Accompanying Short-Term Memory Disturbances in Experimental Models
    AU  - Owolabi Joshua Oladele
    AU  - Olatunji Sunday Yinka
    AU  - Olanrewaju John Afees
    AU  - Jimoh-Enesi Queen Amina
    Y1  - 2017/10/26
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajlm.20170205.15
    DO  - 10.11648/j.ajlm.20170205.15
    T2  - American Journal of Laboratory Medicine
    JF  - American Journal of Laboratory Medicine
    JO  - American Journal of Laboratory Medicine
    SP  - 104
    EP  - 111
    PB  - Science Publishing Group
    SN  - 2575-386X
    UR  - https://doi.org/10.11648/j.ajlm.20170205.15
    AB  - A number of literatures have reported that caffeine could have negative effects on mental health and brain structures and chemistry. There are also reported positive effects of caffeine, such as improvement in memory, especially for those who consume caffeine from its natural sources. Sixty male juvenile Wistar rats were grouped into six (6) groups of 10 rats in each. The groups were labeled A-F with group A being the control group and groups B, C, D, E, and F being the treated groups. This investigation complements our previous efforts to study the effects of caffeine on the choroid plexus microscopic structure, which is involved in the production of the cerebrospinal fluids. Caffeine alters the activities of key enzymes that are associated with the production of CSF. This showed that the structural changes have observable alterations of the brain chemistry. Also, memory, a major attribute of mental power was tested in the same models; results showed that caffeine affected the short term memories. The effects of caffeine was studied in models that ingested caffeine solely and in other modeled after human use by consuming it with honey, a natural antioxidant rich sweetener. In this study, it was observed that the long-term consumption and high amount of caffeine increased the expression of carbonic anhydrase enzyme in the choroid plexus, this enzyme plays a major role in the production of CSF. In the caffeine-treated rats, there was also increased expression of Na+/K+-ATPase which may be associated with changes in the choroid epithelial cells. Honey improved the glutathione peroxidase level. Results showed the use of caffeine with honey had positive effects against the observed effects caused by ingestion of caffeine only. This also showed that caffeine use effects would vary in users of pure caffeine as mere drugs; and habitual consumers as beverages. Generally, evidences lead us to conclude that alterations in enzymes activities are associated with choroid plexus changes that could affect CSF production. These effects are also associated with behavioural changes.
    VL  - 2
    IS  - 5
    ER  - 

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Author Information
  • Department of Anatomy, Babcock University, Ilishan-Remo, Nigeria

  • Department of Anatomy, Babcock University, Ilishan-Remo, Nigeria

  • Department of Anatomy, Babcock University, Ilishan-Remo, Nigeria

  • Department of Anatomy, Babcock University, Ilishan-Remo, Nigeria

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