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Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque

Received: 14 August 2016     Accepted: 14 November 2016     Published: 18 January 2017
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Abstract

This study evaluated the influence of long-term simulated microgravity on oxidative stress and total antioxidant capacity in the plasma and lung tissues of rhesus macaque (-10℃ head-down tilting). Fifteen healthy male rhesus macaques were randomly divided into groups 1 (control, n=5), groups 2 (head-down tilting for 6 weeks, n=5) and groups 3 (head-down tilting for 6 weeks and recover from 4 weeks, n=5). Oxidative stress was evaluated by critical SOD, GSH, H2O2 in plasma and SOD, GSH in lung tissues. HE staining was used to observe the histopathological structure changes of pulmonary tissues. CAT, SOD1, SOD2, SOD3, GPX1, GPX4, GPX7, PRDX1, HMOX1, ALOX5 and DUOX1 mRNA were measured by real-time PCR. GSH concentration was significantly decreased, whereas H2O2 level was significantly increased in group 2 compared with group 1 and group 3. Compared to group 1, histopathological examination revealed alveolar septal thickening, and alveolar and interstitial lymphocytic infiltration in group 2 and group 3 and the pathological changes in group 3 were smaller than those in group 2. Group 2 and group 3 showed significant up-regulation of SOD3 gene compared with group 1 by real-time PCR. In a long-term simulated microgravity environment, systemic antioxidant level of GSH was reduced but an oxidative stress marker of H2O2 was increased. Meanwhile, long-term simulated microgravity caused lung injury and induced the mRNA of SOD3 expression in lung tissues. But oxidant stress is not a major factor involved in the development of lung damage under simulated microgravity. Further study still clarifies the mechanism about the lung injury under microgravity.

Published in American Journal of Laboratory Medicine (Volume 2, Issue 1)
DOI 10.11648/j.ajlm.20170201.11
Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Simulated Microgravity, Oxidative Stress, GSH, H2O2

References
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Cite This Article
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    Yang Chen, Ping Wang, Chongyu Xu, Yiling Cai, Huasong Ma. (2017). Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque. American Journal of Laboratory Medicine, 2(1), 1-6. https://doi.org/10.11648/j.ajlm.20170201.11

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    ACS Style

    Yang Chen; Ping Wang; Chongyu Xu; Yiling Cai; Huasong Ma. Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque. Am. J. Lab. Med. 2017, 2(1), 1-6. doi: 10.11648/j.ajlm.20170201.11

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    AMA Style

    Yang Chen, Ping Wang, Chongyu Xu, Yiling Cai, Huasong Ma. Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque. Am J Lab Med. 2017;2(1):1-6. doi: 10.11648/j.ajlm.20170201.11

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  • @article{10.11648/j.ajlm.20170201.11,
      author = {Yang Chen and Ping Wang and Chongyu Xu and Yiling Cai and Huasong Ma},
      title = {Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque},
      journal = {American Journal of Laboratory Medicine},
      volume = {2},
      number = {1},
      pages = {1-6},
      doi = {10.11648/j.ajlm.20170201.11},
      url = {https://doi.org/10.11648/j.ajlm.20170201.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20170201.11},
      abstract = {This study evaluated the influence of long-term simulated microgravity on oxidative stress and total antioxidant capacity in the plasma and lung tissues of rhesus macaque (-10℃ head-down tilting). Fifteen healthy male rhesus macaques were randomly divided into groups 1 (control, n=5), groups 2 (head-down tilting for 6 weeks, n=5) and groups 3 (head-down tilting for 6 weeks and recover from 4 weeks, n=5). Oxidative stress was evaluated by critical SOD, GSH, H2O2 in plasma and SOD, GSH in lung tissues. HE staining was used to observe the histopathological structure changes of pulmonary tissues. CAT, SOD1, SOD2, SOD3, GPX1, GPX4, GPX7, PRDX1, HMOX1, ALOX5 and DUOX1 mRNA were measured by real-time PCR. GSH concentration was significantly decreased, whereas H2O2 level was significantly increased in group 2 compared with group 1 and group 3. Compared to group 1, histopathological examination revealed alveolar septal thickening, and alveolar and interstitial lymphocytic infiltration in group 2 and group 3 and the pathological changes in group 3 were smaller than those in group 2. Group 2 and group 3 showed significant up-regulation of SOD3 gene compared with group 1 by real-time PCR. In a long-term simulated microgravity environment, systemic antioxidant level of GSH was reduced but an oxidative stress marker of H2O2 was increased. Meanwhile, long-term simulated microgravity caused lung injury and induced the mRNA of SOD3 expression in lung tissues. But oxidant stress is not a major factor involved in the development of lung damage under simulated microgravity. Further study still clarifies the mechanism about the lung injury under microgravity.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque
    AU  - Yang Chen
    AU  - Ping Wang
    AU  - Chongyu Xu
    AU  - Yiling Cai
    AU  - Huasong Ma
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    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajlm.20170201.11
    DO  - 10.11648/j.ajlm.20170201.11
    T2  - American Journal of Laboratory Medicine
    JF  - American Journal of Laboratory Medicine
    JO  - American Journal of Laboratory Medicine
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    EP  - 6
    PB  - Science Publishing Group
    SN  - 2575-386X
    UR  - https://doi.org/10.11648/j.ajlm.20170201.11
    AB  - This study evaluated the influence of long-term simulated microgravity on oxidative stress and total antioxidant capacity in the plasma and lung tissues of rhesus macaque (-10℃ head-down tilting). Fifteen healthy male rhesus macaques were randomly divided into groups 1 (control, n=5), groups 2 (head-down tilting for 6 weeks, n=5) and groups 3 (head-down tilting for 6 weeks and recover from 4 weeks, n=5). Oxidative stress was evaluated by critical SOD, GSH, H2O2 in plasma and SOD, GSH in lung tissues. HE staining was used to observe the histopathological structure changes of pulmonary tissues. CAT, SOD1, SOD2, SOD3, GPX1, GPX4, GPX7, PRDX1, HMOX1, ALOX5 and DUOX1 mRNA were measured by real-time PCR. GSH concentration was significantly decreased, whereas H2O2 level was significantly increased in group 2 compared with group 1 and group 3. Compared to group 1, histopathological examination revealed alveolar septal thickening, and alveolar and interstitial lymphocytic infiltration in group 2 and group 3 and the pathological changes in group 3 were smaller than those in group 2. Group 2 and group 3 showed significant up-regulation of SOD3 gene compared with group 1 by real-time PCR. In a long-term simulated microgravity environment, systemic antioxidant level of GSH was reduced but an oxidative stress marker of H2O2 was increased. Meanwhile, long-term simulated microgravity caused lung injury and induced the mRNA of SOD3 expression in lung tissues. But oxidant stress is not a major factor involved in the development of lung damage under simulated microgravity. Further study still clarifies the mechanism about the lung injury under microgravity.
    VL  - 2
    IS  - 1
    ER  - 

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Author Information
  • Department of Respiratory Medicine, 306th Hospital of PLA, Beijing, China

  • Department of Respiratory Medicine, 306th Hospital of PLA, Beijing, China

  • Department of Respiratory Medicine, 306th Hospital of PLA, Beijing, China

  • Department of Neurology, 306th Hospital of PLA, Beijing, China

  • Department of Orthopedic, 306th Hospital of PLA, Beijing, China

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